Part:BBa_K953003

Agrobacterium tumefaciens Bacteriophytochrome with T7 & RBS (E. coli Codon Optimised)

Agrobacterium tumefaciens bacteriophytochrome with a RBS and codon optimised for expression in E. coli. This part differs from Part BBa_K953002 in that it also includes a T7 promoter (Part BBa_I712074). In the presence of biliverdin the bacteriophytochrome will undergo a conformation change. This complex can then absorb red light (620-750 nm) to excite the bacteriophytochrome and result in a phenotypic change from blue to green. This can be reversed by far-red light (700-800 nm) or will revert from green to blue over time. As E. coli does not produce biliverdin, heme oxygenase (Part BBa_K953004) can be coupled with this part to activate the oxidation of heme to produce biliverdin.

The results and characterisation of bacteriophtyochrome can be found here and you can also check out our entire project be clicking here.

Visualisation of Agrobacterium tumefaciens Bacteriophytochrome with T7 , containing correct bands approximately 2.25kb, thus determining the constructs were successful.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 1559
  Illegal BamHI site found at 1919
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]